生长因子对出芽短梗霉产聚苹果酸的影响

李清1; 王淑贤1; 韩璐瑶1; 王蕊1; 赵廷彬2; 张琳2; 曹伟锋1; 3; 4*; 乔长晟1; 2; 3; 4*

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DOI:10.12161/j.issn.1005-6521.2024.24.004

食品研究与开发:2024,45(24):27-34

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生长因子对出芽短梗霉产聚苹果酸的影响

李清1，王淑贤1，韩璐瑶1，王蕊1，赵廷彬2，张琳2，曹伟锋1，3，4*，乔长晟1，2，3，4*^1,2,3,4

(1.天津科技大学生物工程学院，天津 300457;2.天津慧智百川生物有限公司，天津 300457;3.工业发酵微生物教育部重点实验室暨天津市工业微生物重点实验室，天津 300457;4.天津市微生物代谢与发酵过程控制技术工程中心，天津 300457)

Effect of Growth Factors on Poly Malic Acid Production by Aureobasidium pullulans

LI Qing1，WANG Shuxian1，HAN Luyao1，WANG Rui1，ZHAO Tingbin2，ZHANG Lin2，CAO Weifeng1，3，4*，QIAO Changsheng1，2，3，4*^1,2,3,4

(1.College of Bioengineering，Tianjin University of Science and Technology，Tianjin 300457，China;2.Tianjin Huizhi Baichuan Bioengineering Co.，Ltd.，Tianjin 300457，China;3.Key Laboratory of Industrial Fermentation Microbiology，Ministry of Education and Tianjin Key Laboratory of Industrial Microbiology，Tianjin 300457，China;4.Tianjin Engineering Center of Microbial Metabolism and Fermentation Process Control，Tianjin 300457，China)

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投稿时间：2024-03-02

中文摘要: 聚苹果酸是一种天然可生物降解的聚合物，在医学及其它工业中的应用愈加受到重视，但其生物合成的产量仍然较低。该文主要研究生长因子（胞嘧啶、腺嘌呤、维生素H、吡哆醇和氯化胆碱）对聚苹果酸合成的影响，并揭示其影响机制。研究发现，5 种生长因子中腺嘌呤对出芽短梗霉产聚苹果酸的影响最大，其较适宜的添加浓度为0.5 g/L。添加0.5 g/L 腺嘌呤时聚苹果酸产量从29.4 g/L 提高到39.3 g/L，产量提高33.7%。利用非标记定量技术和液相色谱-串联质谱法分析腺嘌呤对聚苹果酸合成的影响机制。与空白组相比，添加0.5 g/L 腺嘌呤出现398 个差异蛋白质，其中202 个上调蛋白，196 个下调蛋白（差异倍数>1.2，P<0.05）。对差异蛋白进行基因本体（gene ontology，GO）功能富集、（kyoto encyclopedia of genes and genomes，KEGG）通路富集分析和代谢途径分析，结果显示差异蛋白质主要参与糖酵解和三羧酸循环（tricarboxylic acid cycle，TCA）途径。

中文关键词: 腺嘌呤出芽短梗霉聚苹果酸液相色谱-串联质谱法蛋白质组学

Abstract:As a naturally biodegradable polymer，poly malic acid（PMLA）has attracted more and more attention in medicine and other industries.However，PMLA remains low in its biosynthetic yield.This paper studied the effect of growth factors，including cytosine，adenine，vitamin H，pyridoxine，and choline chloride，on the synthesis of PMLA and the underlying mechanisms were revealed.It was found that among the five growth factors，adenine had the greatest effect on PMLA production by Aureobasidium pullulans，with an optimal concentration of 0.5 g/L. By adding 0.5 g/L adenine，the PMLA production went up from 29.4 g/L to 39.3 g/L，an increase of 33.7%.Further，the label-free quantitative method and liquid chromatography-tandem mass spectrometry were used to analyze the mechanism of adenine′s influence on PMLA synthesis.Compared with the control group，the addition of 0.5 g/L adenine resulted in 398 differential proteins，including 202 up-regulated proteins and 196 down-regulated proteins（fold change>1.2，P<0.05）. Through gene ontology（GO）functional enrichment analysis，KEGG pathway enrichment analysis and metabolic pathway analysis of the differential proteins，it could be seen that the differential proteins were mainly involved in glycolysis and tricarboxylic acid cycle（TCA）cycle pathways.

keywords: adenine Aureobasidium pullulans poly malic acid liquid chromatography-tandem mass spectrometry（LC-MS） proteomics

文章编号：202424004 中图分类号： 文献标志码：

基金项目:国家自然科学基金项目（22278412）；宁夏回族自治区重点研发计划项目（2021BEG03011、2022BEG02006）

作者	单位
李清1，王淑贤1，韩璐瑶1，王蕊1，赵廷彬2，张琳2，曹伟锋1，3，4，乔长晟1，2，3，4	1.天津科技大学生物工程学院，天津 300457；2.天津慧智百川生物有限公司，天津 300457；3.工业发酵微生物教育部重点实验室暨天津市工业微生物重点实验室，天津 300457；4.天津市微生物代谢与发酵过程控制技术工程中心，天津 300457

Author Name

Affiliation

LI Qing1，WANG Shuxian1，HAN Luyao1，WANG Rui1，ZHAO Tingbin2，ZHANG Lin2，CAO Weifeng1，3，4*，QIAO Changsheng1，2，3，4*

1.College of Bioengineering，Tianjin University of Science and Technology，Tianjin 300457，China；2.Tianjin Huizhi Baichuan Bioengineering Co.，Ltd.，Tianjin 300457，China；3.Key Laboratory of Industrial Fermentation Microbiology，Ministry of Education and Tianjin Key Laboratory of Industrial Microbiology，Tianjin 300457，China；4.Tianjin Engineering Center of Microbial Metabolism and Fermentation Process Control，Tianjin 300457，China

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