Abstract:The proliferation and enzyme production of Staphylococcus xylosus TG022 treated with polysaccharides from five polysaccharides （Taraxaci Herba,Astragali Radix,Lycii Fructus,Dioscoreae Rhizoma,and Angelicae Sinensis Radix） with both edible and medicinal values were studied.The regulating effects of Taraxaci Herba polysaccharide and Angelicae Sinensis Radix polysaccharide combined with S.xylosus TG022 on intestinal flora were explored.The five polysaccharides were respectively used as the sole carbon sources for the culture of S.xylosus TG022,and the polysaccharides suitable for strain growth was screened out based on the growth curve,pH,and enzyme activity.Furthermore,in vitro intestinal fermentation experiments were carried out to examine the effects of the polysaccharides screened out on intestinal flora.The results showed that Angelicae Sinensis Radix polysaccharide and Taraxaci Herba polysaccharide had the best effects on the growth of S.xylosus TG022,increasing the biomass and enzyme activity.The polysaccharides from the other three Chinese herbal medicines had no promoting effect on the growth of S.xylosus TG022.The results of 16S rRNA gene high-throughput sequencing showed that compared with the negative control,fermentation of S.xylosus TG022 with Angelicae Sinensis Radix or Taraxaci Herba polysaccharide reduced the relative abundance of pathogenic microorganisms and increased the relative abundance of beneficial microorganisms.Angelicae Sinensis Radix or Taraxaci Herba polysaccharide could be used as the only carbon source to promote the growth of S.xylosus TG022,promoting the proliferation,enzyme production,and in vitro intestinal fermentation.S.xylosus TG022 combined with each of the two polysaccharides can regulate the relative abundance of pathogenic microorganisms and beneficial microorganisms in the intestine.