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投稿时间：2023-09-12
投稿时间：2023-09-12
中文摘要: 为探究杏鲍菇多糖（Pleurotus eryngii polysaccharides，PEP）对CCl4 诱导小鼠急性肝损伤的干预作用，首先分析PEP 体外清除DPPH 自由基及ABTS+自由基的能力，之后将小鼠随机分为对照组、模型组、PEP 低、中、高剂量组（100、200、400 mg/kg），连续灌胃干预21 d 后，腹腔注射CCl4 以诱导小鼠急性肝损伤，最后测定小鼠血清谷丙转氨酶（alanine transaminase，ALT）、谷草转氨酶（aspartate transaminase，AST）活性、总胆红素（total bilirubin，TBIL）、总胆固醇（total cholesterol，TC）、甘油三酯（triglyceride，TG）、肿瘤坏死因子-α（tumor necrosis factor-α，TNF-α）、白细胞介素-6（interleukin-6，IL-6）含量，分析肝组织丙二醛（malondialdehyde，MDA）含量、过氧化氢酶（catalase，CAT）、超氧化物歧化酶（superoxide dismutase，SOD）、谷胱甘肽过氧化物酶（glutathione peroxidase，GSH-Px）活性并检测肝脏组织形态学变化。结果表明，PEP 对ABTS+自由基及DPPH 自由基清除率分别可达（70.50±0.63）%和（63.25±0.56）%。与模型组比较，PEP 各剂量组肝脏、肾脏及脾脏指数呈不同程度降低。血清ALT、AST 活性、TBIL、TC、TG、TNF-α、IL-6 含量显著下降，肝组织SOD、CAT、GSH-Px 活性显著增高，MDA 含量显著下降（P＜0.05），且呈剂量依赖性。PEP 各剂量组肝组织病理损伤不同程度减轻。因此PEP 能够通过抗氧化及抗炎对CCl4 所致的小鼠肝损伤发挥保护作用。
Abstract:To analyze the intervention effect of Pleurotus eryngii polysaccharides（PEP）on carbon tetrachloride（CCl4）-induced acute liver injury in mice，the scavenging ability of PEP against DPPH free radicals and ABTS+ free radicals in vitro was analyzed. Then，the mice were randomized into the control group，model group，low-，medium-，and high-dose PEP groups（100，200，400 mg/kg）.After continuous gavage intervention for 21 d，CCl4 was injected intraperitoneally to induce acute liver injury in mice. Finally，the activities of serum alanine transaminase（ALT）and aspartate transaminase（AST），and the contents of total bilirubin（TBIL），total cholesterol（TC），triglyceride（TG），tumor necrosis factor-α（TNF-α）and interleukin-6（IL-6）were measured，the malondialdehyde（MDA）content and the activities of catalase（CAT），superoxide dismutase（SOD）and glutathione peroxidase（GSH-Px）in the liver tissues were analyzed，and the morphological changes in the liver tissues were detected. The results showed that the scavenging rates of PEP against ABTS+ free radicals and DPPH free radicals were（70.50±0.63）% and（63.25±0.56）%，respectively. Compared with the model group，the liver，kidney，and spleen indexes of each PEP group decreased to varying degrees.The serum levels of ALT，AST，TBIL，TC，TG，TNF-α and IL-6 decreased significantly，the activities of SOD，CAT，and GSH-Px in the liver tissues significantly increased，and the content of MDA decreased significantly（P＜0.05），in a dose-dependent manner. Pathological damage of liver tissues in each of the PEP groups was alleviated to varying degrees.Therefore，PEP could play a protective role in CCl4-induced liver injury in mice through anti-oxidant and anti-inflammatory effects.
keywords: Pleurotus eryngii polysaccharide carbon tetrachloride acute liver injury protective effect
文章编号：202421010 中图分类号： 文献标志码：
基金项目:新疆维吾尔自治区自然科学基金项目（2022D01B158）
| 作者 | 单位 |
| 李惠琳1，2，李珏1，2，李宝通1，艾则麦提·图尔洪1，赵雷1，黄中杰1，何庆峰1，2* | 1.和田职业技术学院农业科技系，新疆 和田 848000；2.天津农学院食品科学与生物工程学院，天津 300384 |
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