Abstract:Obesity has become a modern epidemic affecting human lifestyles around the world.Inhibiting the activity of pancreatic lipase is the key to the treatment of obesity.In this study，the bioaccessibility and antiobesity properties of polyphenols from black tea after in vitro simulated digestion were investigated，and the inhibitory mechanism of theaflavin-3，3′-digallate（TFDG）on pancreatic lipase activity was explored by fluorescence spectroscopy.The results of in vitro simulated digestion experiments showed that the phenolic content of black tea decreased，and the final bioaccessibility was maintained at 32.04%.However，after in vitro digestion，the inhibition rate of black tea on pancreatic lipase increased by 26.97%，which effectively inhibited the hydrolysis of dietary lipid.The results of fluorescence analysis showed that TFDG could statically quench the intrinsic fluorescence of pancreatic lipase.Hydrogen bonding and hydrophobic interaction were the main driving forces in the binding process of the two，and the conformation of pancreatic lipase had changed in the binding process.