Abstract:Bioactive polysaccharide was extracted from Citrus maxima peel with enzymatic hydrolysis to improve the utilization rate of C.maxima.The amount of enzyme，the liquid-to-solid ratio，and the extraction time in the enzymatic hydrolysis extraction process were optimized by the Box-Behnken design with the polysaccharide yield as the indicator.The results indicated that the optimal extraction conditions were extraction at the liquid-to-solid ratio of 20∶1（mL/g）and enzyme addition amount of 1.0 g for 2 h.Under these conditions，the yield of polysaccharide from C.maxima peel reached up to（2.73±0.21）%.Enzymatic hydrolysis can effectively improve the extraction rate of polysaccharide from C.maxima peel.