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投稿时间：2023-03-22
投稿时间：2023-03-22
中文摘要: 采用“52×3 双水相试验法”优化双水相萃取条件，分别探究鲍鱼内脏、扇贝内脏和生蚝内脏双水相下相萃取物中的β-葡萄糖苷酶的酶活性和萃取效果。结果表明，采用该试验方法优化所得的临界体系为12% PEG4000/15% NaH2PO4，以该体系萃取获得的下相萃取物中β-葡萄糖苷酶在酶活力、酶比活、纯化倍数和酶活回收率大小为生蚝内脏＞鲍鱼内脏＞扇贝内脏。
中文关键词: 鲍鱼内脏 52×3 双水相试验法 临界萃取 β-葡萄糖苷酶 萃取效果
Abstract:The enzyme activity and extraction effect of β-glucosidase in the aqueous two-phase subphase extracts of abalone viscera，scallop viscera and oyster viscera were investigated by using “52×3 aqueous two-phase extraction method” to optimize the extraction conditions.The results showed that the critical system optimized by this method was 12% PEG4000/15% NaH2PO4.The β-glucosidase of subphase extracts extracted by this system showed the trend of oyster viscera>abalone viscera>scallop viscera in terms of enzyme activity，specific activity，purification ratio，and recovery.
keywords: abalone viscera 52×3 aqueous two-phase extraction method critical extraction β-glucosidase extraction effect
文章编号：202316014 中图分类号： 文献标志码：
基金项目:厦门医学院“大学生创新创业训练计划”资助项目（X202212631020）
| Author Name | Affiliation |
| ZENG Ying，ZENG Shuxin，HE Yushu，LIN Yunqi | Xiamen Medical College，Xiamen 361021，Fujian，China |
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