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食品研究与开发:2023,44(8):191-200
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酱香型白酒酿造过程中高温放线菌的筛选及其特性
(1.河北科技大学食品与生物学院,河北 石家庄 050000;2.北京工商大学,北京食品营养与人类健康高精尖创新中心,北京 100048;3.北京华都酿酒食品有限责任公司,北京 102212)
Screening and Characterization of Thermophilic Actinomycetes Isolated from Sauce-flavor Baijiu
(1.College of Food Science and Biology,Hebei University of Science and Technology,Shijiazhuang 050000,Hebei,China;2.Beijing Technology and Business University,Beijing Top Innovation Center for Food Nutrition and Human Health,Beijing 100048,China;3.Beijing Huadu Brewery&Food Industry Co.,Ltd.,Beijing 102212,China)
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投稿时间:2022-01-04    
中文摘要: 为探究白酒酿造过程中高温放线菌的特性,利用传统方法从酱香型白酒酿造过程中筛选得到3株高温放线菌,经形态学、16S rDNA生物学鉴定、生理生化及特性分析,确定分离纯化出的F-40号菌株为白色链霉(Streptomyces albus)、F-41号菌株为橄榄链霉菌(Streptomyces olivaceus)、F-42号菌株为植物内生糖多孢菌(Saccharopolyspora endophytica)。F-40号菌株的最适生长温度为30℃,最适生长pH8,最大耐乙醇浓度为9%;F-41号菌株的最适生长温度为50℃,最适生长pH7,最大耐乙醇浓度为6%;F-42号菌株的最适生长温度为40℃,最适生长pH6,最大耐乙醇浓度为6%。3株放线菌代谢产生其各自的特征性风味物质,F-40号菌株可代谢产生反-萘烷醇,F-41号菌株可代谢产生小分子醇类及内酯,F-42号菌株可代谢产生不饱和醛类,且均可代谢产生较多的醇类及醛酮类物质,如2-庚酮、苯乙醇等,有助于酱酒风味的形成。
Abstract:To explore the characteristics of thermophilic actinomycetes in liquor making,three strains of actinomycetes—namely,F-40,F-41,and F-42—were screened from traditional sauce-flavor Baijiu.Based on the results,F-40,F-41,and F-42 were identified as Streptomyces albus,Streptomyces olivaceus,and Saccharopolyspora endophytica,respectively.The optimum growth temperature,pH,and maximum alcohol tolerance of the strain F-40 were 35 ℃,8,and 9%,respectively;those of the strain F-41 were 50 ℃,7,and 6%,respectively;and those of the strain F-42 were 40 ℃,6,and 6%,respectively.The three strains used different metabolic processes to produce their own characteristic flavor substances.Special trans-naphthanol,small molecular alcohols and lactones,and unsaturated aldehydes were detected in the fermentation samples of the strains F-40,F-41,and F-42,respectively.These compounds could be metabolized to produce more alcohols and aldehydes and ketones,such as 2-heptanone,which is conducive to the formation of the soy sauce flavor.This study aims to provide data related to the flavor of Baijiu.
文章编号:202308028     中图分类号:    文献标志码:
基金项目:国家自然科学基金项目(31830069、31901632);国家重点研发计划项目(2022YFD2101401)
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