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投稿时间:2022-09-13
投稿时间:2022-09-13
中文摘要: 以绿豆为原料,研究绿豆多糖最佳提取工艺,在单因素试验的基础上,利用Box-Behnken 响应面分析法优化超声辅助复合酶法提取绿豆多糖工艺,同时探究其功能特性。结果表明:绿豆多糖的最佳提取工艺为水提温度95 ℃、水提时间186 min、超声时间32 min、料液比1∶22(g/mL),在此条件下,多糖得率为5.77%。绿豆多糖功能特性测定结果显示,绿豆多糖持水性2.89 mL/g、持油性3.07 mL/g、乳化性14.69%、乳化稳定性47.36%、起泡性34.32%、泡沫稳定性24.16%,绿豆多糖展现出较强的自由基清除能力,对DPPH·、·OH 和O2-·清除率最高为78.41%、88.32%和52.66%。
Abstract:This study aims to optimize the extraction process of mung bean polysaccharides.On the basis of single factor test,Box-Behnken design was used to optimize the ultrasound-assisted extraction process of mung bean polysaccharides,and the functional characteristics were discussed. The results showed that the optimum extraction conditions were as follows:water extraction temperature of 95 ℃,water extraction time of 186 min,ultrasonic time of 32 min,and solid-to-liquid ratio of 1∶22 (g/mL). Under the optimum conditions,the yield of polysaccharides was 5.77%. The polysaccharides showed the water-holding capacity,oil-holding capacity,emulsifying capacity,emulsification stability,foaming capacity,and foam stability of 2.89 mL/g,3.07 mL/g,14.69%,47.36%,34.32% and 24.16% respectively.The mung bean polysaccharides showed strong free radicalscavenging activity,and the highest DPPH·-,·OH-,and O2-·-scavenging rates were 78.41%,88.32% and 52.66%,respectively.
keywords: polysaccharides mung bean response surface methodology functional characteristics antioxidant activity
文章编号:202306010 中图分类号: 文献标志码:
基金项目:国家自然科学基金项目(32172288);河北省现代农业产业技术体系杂粮杂豆产后加工岗位专项(HBCT2018070206);河北农业大学食品加工学科群经费资助项目(2021-06)
作者 | 单位 |
王文韬1,徐慧2,张蕴哲1,苑宁2,卢鑫2,张伟1,3* | 1.河北农业大学 食品科技学院,河北 保定 071001;2.河北农业大学 理工系,河北 沧州 061100;3.河北省人畜共患病原微生物分析与防控重点实验室,河北 保定 071000 |
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