Abstract:To study the effects of cold plasma treatment conditions on protein and lipid oxidation of Trachinotus ovatus，treatment time，voltage，treatment times，post-storage time，and treatment styles were taken as main factors for cold plasma treatment.The protein carbonyl，total sulfhydryl，protein surface hydrophobicity，and thiobarbituric acid reactive substances（TBARS）values were measured and analyzed before and after treatment.The results showed that when the treatment voltage was increased and the treatment time was prolonged，the cold plasma increased the carbonyl content，protein surface hydrophobicity and TBARS values of T.ovatus，and reduced its sulfhydryl content.When the total treatment time was 180 s and treatment times were 2-4，the degree of fish lipid and protein oxidation was significantly lower than that of the treatment once.These results indicated that high treatment voltage，long treatment time，and multiple cold plasma treatment times would promote the oxidation of lipids and proteins in T.ovatus，and prolonging the storage time after treatment could also accelerate the oxidation of samples.Therefore，controlling and optimizing treatment conditions were the key to promote the application of cold plasma in fresh keeping of T.ovatus.