Abstract:The aim of this study was to evaluate the ameliorating effects of Perilla frutescens seeds polysaccharide on abnormal lipid metabolism and oxidative stress in mice treated with a high-fat diet.One hundred and fifty healthy Kunming mice were selected and randomly divided into control，high-fat（HF）diet，low-dose Perilla frutescens seeds polysaccharide（L-PFSP），medium-dose Perilla frutescens seeds polysaccharide（M-PFSP），and high-dose perilla frutescens seeds polysaccharide（H-PFSP）groups.The results showed that，at the end of the 8-week experimental period，mice in the HF diet group weighed significantly more than those in the control group.Dietary supplementation with Perilla frutescens seeds polysaccharide（M-PFSP and H-PFSP groups）significantly decreased liver index values and serum aspartate aminotransferase （AST），alanine aminotransferase，triglyceride（TG），total cholesterol（TC），low-density lipoprotein-C，and glucose levels in mice fed an HF diet，and significantly increased serum high-density lipoprotein-C levels.Moreover，the addition of Perilla frutescens seeds polysaccharide（M-PFSP and H-PFSP groups）significantly reduced TC and TG levels in the liver，and increased the levels of superoxide dismutase and peroxidase in the liver and intestine.Catalase and glutathione peroxidase activities and malondialdehyde levels in the liver and intestine were also significantly reduced.The results of mRNA and western blotting analyses showed that Perilla frutescens seeds polysaccharide significantly reduced the mRNA and protein expression levels of fatty acid synthetase in the liver of mice fed an HF diet，and significantly increased carnitine palmitoyltransferase I and adipose triglyceride lipase mRNA and protein expression levels.In conclusion，Perilla frutescens seeds polysaccharide alleviated oxidative stress and abnormal lipid metabolism in mice fed an HF diet.