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投稿时间：2021-03-01
投稿时间：2021-03-01
中文摘要: 以海藻酸钠为载体，包埋法固定化短乳杆菌，催化L-谷氨酸钠合成γ-氨基丁酸，通过正交试验对细胞固定化条件进行优化，并对固定化细胞的酶学性质及操作稳定性进行研究。确定最佳固定化条件为海藻酸钠浓度2.5%、CaCl2浓度3.0%、固定化细胞平均粒径3.2 mm、硬化时间4 h。在此条件下制备的固定化细胞最适酶反应pH值为4.5，最适温度为35℃，且表现出较好的操作稳定性，重复利用至第7次后仍保持53.2%的相对酶活力。
Abstract:Lactobacillus brevis was immobilized on sodium alginate by embedding method to catalyze the synthesis of γ-aminobutyric acid from sodium L-glutamate.The conditions for cell immobilization were optimized by orthogonal test，and the enzymatic properties and operational stability of the immobilized cells were studied.The conditions for cell immobilization were optimized as 2.5% sodium alginate，3.0% CaCl2，average particle size of 3.2 mm and immobilization for 4 h.The cells immobilized under the above conditions presented the best performance at pH 4.5 and 35℃.The immobilized cells showed well operational stability and maintained 53.2% relative enzyme activity after reused seven times.
keywords: γ-aminobutyric acid Lactobacillus brevis sodium alginate immobilized cells embedding method
文章编号：202211024 中图分类号： 文献标志码：
基金项目:辽宁省自然科学基金面上项目（2019-MS-044）
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