Abstract:Chimonobambusa quadrangularis（Fenzi）Makino leaves were chosen as raw material.The four extraction methods，included ethanol extraction，cellulase extraction，ultrasound assisted ethanol extraction and vibration assisted ethanol extraction were evaluated by compared the extraction rate of flavonoids，and the optimal extraction process of flavonoids from Chimonobambusa quadrangularis（Fenzi） Makino leaves was obtained by the response surface optimization experiment.At the same time，the anti-oxidant ability of the extracts of Chimonobambusa quadrangularis（Fenzi）Makino leaves obtained by the four extraction methods was evaluated.The results showed that the flavonoids extracted with ultrasonic assisted ethanol had a high extraction rate up to（4.06±0.06）%.The optimum extraction conditions were as follows：the ratio of solid to liquid was 1∶25（g/mL），the ethanol concentration was 80%，ultrasonic time was 112 min，ultrasonic temperature was 57 ℃，ultrasonic power was 85 W.With the increase in the mass concentration of the flavonids of Chimonobambusa quadrangularis（Fenzi）Makino leaves，the DPPH free radical scavenging ability and Fe3+reduction ability of the flavonids by different extraction methods were gradually enhanced.The antioxidant capacity of the flavonids by ultrasonic assisted ethanol extraction were the strongest.