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投稿时间：2019-05-29
投稿时间：2019-05-29
中文摘要: 建立可同时测定红糖中酒石酸、丙酮酸、乙酸、柠檬酸、琥珀酸、没食子酸以及乌头酸含量的固相萃取-高效液相色谱法。结果发现当检测器为二极管阵列紫外-可见光检测器，检测波长210 nm，XBridge C8色谱柱（250 mm×4.6 mm，5 μm），柱温为35℃，流动相为0.1%磷酸∶乙腈溶液=99∶1，流速为0.7 mL/min，红糖中的7种有机酸能在15 min内均达到基线分离。检测限分别在0.005 2 mg/kg～0.77 mg/kg之间，标准曲线相关系数为0.999 6～1.000，精密性试验相对标准偏差值为1.1%～3.0%，样品的回收率在82.1%～108.6%之间。由此可见，此方法适用于快速测定红糖中7种有机酸含量。
Abstract:A method for the determination of 7 kinds of organic acids such as tartaric acid，pyruvic acid，acetic acid，citric acid，succinic acid，gallic acid，and aconitic acid in brown sugar was established by high performance liquid chromatography（HPLC）.The chromatographic conditions were as follows：the mobile phase was 0.1%phosphoric acid ∶acetonitrile=99 ∶1，XBridge C8column （250 mm×4.6 mm，5 μm），column temperature was 35℃，flow rate was 0.7 mL/min，detection wavelength was 210 nm，7 kinds of organic acids were simultaneous separated under the optimized conditions within 15 minutes.The detection limit was between 0.005 2 mg/kg-0.77 mg/kg，the correlation of the standard curve was between 0.999 6-1.000，the relative standard deviation was 1.1%-3.0%，and the recoveries were between 82.1%-108.6%.Thus，it could be seen that this method was suitable for the rapid determination of 7 kinds of organic acid content in brown sugar.
keywords: solid-phase extraction high performance liquid chromatography organic acid brown sugar chromatographic optimization
文章编号：202011029 中图分类号： 文献标志码：
基金项目:广州市科技计划项目（201806010093）
| 作者 | 单位 |
| 陈其钊，陈嘉敏，陈红香，王桂华，李家威，余构彬 | 广东省生物工程研究所（广州甘蔗糖业研究所）广东省甘蔗改良与生物炼制重点实验室，广东广州510316；国家糖业质量监督检验中心，广东广州510316 |
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