Abstract:To establish a pretreatment method for detecting aflatoxins in food without using immunoaffinity columns，a double-layer ionic liquid effervescent tablet was prepared. Under aqueous extraction conditions，this tablet was used to assist in the extraction of target substances from samples. The results showed that the ionic liquid effervescent tablet could efficiently and rapidly transfer aflatoxins from the sample solution into the ionic liquid，enabling qualitative and quantitative detection when combined with high-performance liquid chromatography-tandem mass spectrometry （HPLC-MS/MS）. The recoveries of the four aflatoxins ranged from 90.33% to 99.02%，with relative standard deviations below 4.07%，and a detection limit of 0.02 ng/g. This method is highly efficient，accurate，sensitive，and broadly applicable. It is suitable for detecting aflatoxins in grains，oils，and dried fruits，and features simple operation，short experimental time，and low reagent consumption.