Abstract:To optimize the enzymatic extraction of Hippophae thamnoides pigments，this study employed response surface methodology to optimize the enzymatic extraction process and investigated the stability of the pigments. The results showed that with the enzymatic hydrolysis time of 121 min，the temperature of 52 ℃，the liquid-to-material ratio of 11.1∶1 （mL/g），and the pH of 5.83，the absorbance content of H. thamnoides pigments was 1.672，which was consistent with the predicted value from the model. The stability tests showed that oxidizing and reducing agents，as well as Fe3+，had significant effects on pigment stability，while light exposure caused structural damage to the pigments. In contrast，Al3+ exhibited a relatively minor impact on pigment stability.