Abstract:The agar well diffusion assay for broad-spectrum bacteriostatic testing involves a large workload and is inefficient. The petri dish partitioning assay is an improved method based on the agar well diffusion assay，designed to enhance the efficiency of broad-spectrum bacteriostatic testing. In this study， the bacteriostatic properties of two bacteriocin-producing lactic acid bacteria against six indicator bacteria were tested using both the agar well diffusion assay and the petri dish partitioning assay. The consistency of the results from the two methods was compared and analyzed by measuring the inhibition zone diameters （agar well diffusion assay）and the inhibition annular sector widths （petri dish partitioning assay）. Under the same test conditions， the culture supernatant of Lactiplantibacillus plantarum CICC24194 exhibited inhibitory effects against all six indicator bacteria. The bacteriostatic activity was as follows： Listeria monocytogenes > Staphylococcus aureus > Shigella > Cronobacter > Salmonella > Escherichia coli. The culture supernatant of Enterococcus faecium CICC24203 also inhibited all six indicator bacteria， with the bacteriostatic activity ordered as： L. monocytogenes > Cronobacter > Shigella > Salmonella > S. aureus > E. coli. The results of broad-spectrum bacteriostatic testing by the petri dish partitioning assay were consistent with those by the agar well diffusion assay. The petri dish partitioning assay allowed for the simultaneous testing of six indicator bacteria in one set of petri dishes，providing accurate， reliable， and stable results， making it suitable for efficient broad-spectrum bacteriostatic testing.