Abstract:To study the preventive and therapeutic effects of functional rice flour on hyperlipidemia induced by high fat in mice and to explore its underlying mechanisms， a mixture of homologous medicinal and edible ingredients such as cassis seeds， kudzu root， Chinese yam， coix seed， and sea buckthorn was combined with buckwheat and quinoa to produce the rice flour， and a hyperlipidemia model was established using a high-fat diet on 48 six-week-old specific pathogen-free healthy male ICR mice， which were administered the functional rice flour by gavage as an intervention during the modeling period. The changes in body weight and liver weight， the levels of serum triglycerides （TG）， total cholesterol （TC）， high-density lipoprotein cholesterol（HDL-C）， and low-density lipoprotein cholesterol （LDL-C）， and the content of total bile acid in serum，liver， and feces of mice in each group were measured. In addition， the expression levels of farnesoid X receptor （FXR）， small heterodimer partner （SHP）， and cytochrome P450 family member 7A1 proteins in liver tissue were detected， and the structure and diversity of the gut microbiota were also analyzed. The results indicated that the functional rice flour effectively reduced the content of TG， TC， and LDL-C in the serum of hyperlipidemic mice， and increased the content of HDL-C； it also decreased the total bile acid content in serum and liver， and increased the total bile acid content in feces； simultaneously， it downregulated the expression of FXR and SHP proteins in the liver， and enhanced the richness and diversity of gut microbiota， improving the gut microbiota structure. Therefore， functional rice flour can prevent and treat hyperlipidemia induced by high fat and regulate the gut microbiota， with a mechanism possibly related to the modulation of the FXR-SHP pathway and the promotion of bile acid excretion.