Abstract:To understand the nutritional parameters and overall differences of fresh donkey milk in large-scale donkey farms in Ulanqab,this paper collected ten fresh donkey milk samples from three pastures,and tested the content of major components,minerals,vitamins,amino acids,and fatty acids. Meanwhile,statistical comparisons and principal component analysis （PCA） were conducted on conventional components,amino acids,and fatty acids. The results show that the protein and fat content in fresh donkey milk from Farm A is the highest,while the lactose and ash content in fresh donkey milk from Farm B is higher,with significant differences compared to the other two farms （P<0.05）. Additionally,β-casein and α-lactalbumin content in the fresh donkey milk protein from Farm A is the highest,with the highest calcium and potassium content and higher vitamin C content among the five major elements. PCA analysis showed that there were significant differences in the composition of samples from different pastures. Moisture,protein,and fat correlated with Gly,undecanoic acid （C11:0）,pentadecanoic acid （C15:0）,heptadecanoic acid （C17:0）,and docosaenoic acid [C20:0（n-6）].Meanwhile,the ash content and lactose correlated with amino acids （Asp,Glu,Val,Met,Ile,Leu,Tyr,Phe and Lys） and low fatty acids [C6:0,C10:0,C12:0,C14:0 （n-5）,C16:0,C18:0 （n-6）,C18:0 （n-9）,C20:0 （n-9）,and taurine]. Therefore,the fresh donkey milk from Farm B has a variety of flavor compounds and the best flavor.