Abstract:The anti-oxidative stress mechanism of Bupleuri Radix was studied by network pharmacology and molecular docking. The major anti-oxidative stress components and core targets of Bupleuri Radix and the common targets and signaling pathways shared by Bupleuri Radix and oxidative stress were obtained by network pharmacological methods. The binding affinity of major components to core target proteins was predicted. The results showed that 14 anti-oxidative stress components and 378 targets were screened from Bupleuri Radix,and 191 common targets were shared by Bupleuri Radix and oxidative stress（1 934 targets）. Gene Ontology（GO） function and Kyoto Encyclopedia of Genes and Genomes （KEGG） pathway analyses showed that Bupleuri Radix mainly regulated oxidative stress via the cancer pathway,Alzheimer′s disease pathway,phosphatidylinositol-3-kinase/protein kinase B signaling pathway,Rap1 signaling pathway,and interleukin-17 signaling pathway. The molecular docking results showed that the main anti-oxidative stress components of Bupleuri Radix exhibited good binding affinity to the core targets related to oxidative stress. In conclusion,Bupleuri Radix might regulate oxidative stress via multiple components,targets,and pathways.