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食品研究与开发:2025,46(7):49-56
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酶法改性对绿豆皮膳食纤维理化性质及其降血糖活性的影响
(1. 上海市农业科学院 作物育种栽培研究所 农产品保鲜加工研究中心,上海 201403;2.上海海洋大学食品学院,上海 201306;3.上海理工大学 健康科学与工程学院,上海 200093;4.上海原野蔬菜食品有限公司,上海 201409)
Effects of Enzymatic Modification on the Physicochemical Properties of Dietary Fiber from Mung Bean Skin and Its Hypoglycemic Activity
(1. Agricultural Products Preservation and Processing Research Center, Crop Breeding & Cultivation Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China;2. College of Food Sciences & Technology, Shanghai Ocean University, Shanghai 201306, China;3. School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China;4. Shanghai Original Wild Vegetable Food Co., Ltd., Shanghai 201409, China)
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投稿时间:2023-11-27    
中文摘要: 为进一步开发利用绿豆皮,提升其营养价值,以绿豆皮为材料,比较两种处理方式(单一酶和复合酶法)对绿豆皮膳食纤维组成、结构、理化性质和体外降血糖活性的影响。复合酶改性组的可溶性膳食纤维(soluble dietary fiber,SDF)含量高于单一酶处理组,在纤维素酶和木聚糖酶添加量 (C120+X120 组)均为120 U/g 时,SDF 含量高达7.56%。酶解改性后,绿豆皮膳食纤维呈现粗糙、多孔、多褶皱状。相比单一酶改性,复合酶解改性后,膳食纤维的持水力、持油力、阳离子交换能力等理化特性提高1~2 倍,吸附葡萄糖能力和α-淀粉酶抑制率也高于单一酶改性组,其中α-淀粉酶抑制率超过60%。C120+X120 组的理化性质和体外降血糖活性最佳。相比单一酶改性组,复合酶改性处理绿豆皮膳食纤维是一种获得SDF 的较好处理方式。
Abstract:To further develop and utilize mung bean skin and enhance its nutritional value, mung bean skin was used as a material to compare the effects of two treatments (single enzyme and composite enzyme methods) on the composition, structure, physicochemical properties, and in vitro hypoglycemic activity of its dietary fibers. The soluble dietary fiber (SDF) content in the composite enzyme-modified group was higher than that in the single enzyme-treated group. In the cellulase and xylanase-treated group (C120 + X120), with both enzymes added at 120 U/g, the SDF content reached 7.56%. After enzymatic modification, the dietary fiber of mung bean skin displayed a rough, porous, and pleated structure. Compared with single enzyme modification,the composite enzyme modification increased the physicochemical properties of the dietary fibers, such as water holding capacity, oil holding capacity, and cation exchange capacity, by approximately 1-2 times. Additionally, the glucose adsorption capacity and α-amylase inhibition rate were higher in the composite enzymemodified group, with the α-amylase inhibition rate exceeding 60%. The C120 + X120 group demonstrated the best physicochemical properties and in vitro hypoglycemic activity. Therefore, compared to the single enzymemodified group, composite enzyme modification is a better method for obtaining SDF from mung bean skin dietary fibers.
文章编号:202507007     中图分类号:    文献标志码:
基金项目:上海市农产品保鲜加工专业技术服务平台(21 DZ2292200);上海农产品保鲜加工工程技术研究中心(19 DZ2251600)
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