Abstract:A metabolomics approach was employed to investigate the changes in blood metabolites in mice with β-lactoglobulin（βlg）-induced allergic reactions following treatment with the epigallocatechin gallate（EGCG）-βlg complex.Potential biomarkers related to allergic reactions and desensitization were screened，and their metabolic pathways were further analyzed to explore the metabolic mechanism of EGCG in βlg desensitization.Mice were treated with a complex of the polyphenolic components EGCG and βlg，aimed at desensitizing them to βlg-induced allergic reactions，and they were used as the model group.The allergic behavioral signs of the blank group，βlg group，and βlg-EGCG group were observed. Based on ultra performance liquid chromatography-quadrupole-electrostatic field orbitrap mass spectrometry（UPLC-QE-MS）technology，metabolomics analysis was conducted on mouse serum. Further，principal component analysis and partial least squares discriminant analysis were used to analyze the differences in metabolic profiles among groups and screen for differential metabolites. The results showed significant differences in metabolic profiles between the βlg group and the βlg-EGCG group.A total of 40 differential metabolites and seven major metabolic pathways related to allergic reactions were identified. It was speculated that the mechanisms underlying βlg sensitization and EGCG desensitization might involve processes such as amino acid metabolism and lipid metabolism.