Abstract:Flammulina velutipes，Auricularia auricular，and Tremella fuciformis were used as raw materials for the extraction of fungal polysaccharides.The antioxidant and antimicrobial activities of different polysaccharides were determined and compared. The scavenging abilities of fungal polysaccharides from the three edible mushrooms against hydroxyl（·OH），superoxide anion（O2-·），1，1-diphenyl-2-picrylhydrazyl（DPPH），and 2，2′-azino-bis（3-ethylbenzothiazoline-6-sulfonic acid）（ABTS+）free radicals were measured.The antimicrobial activities of the three polysaccharides against Escherichia coli，Staphylococcus aureus，Bacillus cereus，and Bacillus subtilis were measured by the filter paper method. The results showed that the three fungal polysaccharides exhibited scavenging abilities against the four free radicals and strong antioxidant activity within the test concentration ranges. F. velutipes polysaccharides had the strongest scavenging ability against DPPH free radicals，with the highest scavenging rate over 90%. A. auricula polysaccharides demonstrated stronger scavenging abilities against superoxide anion and ABTS+ free radicals than the other two polysaccharides. All three polysaccharides exhibited hydroxyl radical scavenging rates over 95%. The inhibitory effects of all the fungal polysaccharides on E. coli and S. aureus enhanced with the increase in the polysaccharide concentration.Among these polysaccharides，F.velutipes polysaccharides had the strongest inhibitory effect on bacteria.In conclusion，the three fungal polysaccharides had strong antioxidant and antimicrobial activities，serving as candidate food antioxidants and preservatives.