Abstract:The diversity and correlation of metabolites in Ophiopogonis Radix and Rhodiolae crenulatae Radix et Rhizoma were studied. Liquid chromatography-high-resolution mass spectrometry was employed to analyze the secondary metabolites in the extracts of Ophiopogonis Radix， Rhodiolae crenulatae Radix et Rhizoma， and their combination. The types and content of the compounds were determined， and principal component analysis （PCA）， partial least squares discriminant analysis（PLS-DA）， and metabolic pathway analysis were performed. The results showed that the metabolites of Rhodiolae Crenulatae Radix et Rhizoma and Ophiopogonis Radix mainly included ketone acids， alcohol phenols， and flavonoids. In addition， Rhodiolae crenulatae Radix et Rhizoma contained abundant glycosides， while Ophiopogonis Radix contained a variety of sugars. There were significant differences in the secondary metabolites in the extracts of Rhodiolae crenulatae Radix et Rhizoma， Ophiopogonis Radix， and their combination. The enrichment analysis showed that the common metabolic pathways of Rhodiolae crenulatae Radix et Rhizoma and Ophiopogonis Radix were riboflavin metabolism，arachidonic acid metabolism， steroid hormone synthesis， starch and sucrose metabolism， fructose and mannose metabolism， and alpha linolenic acid metabolism. The common metabolic pathways provided a basis for the discovery of small molecule compounds with antioxidant， anti-inflammatory， yin-nourishing， and immunityenhancing effects in root and rhizoma medicinal materials. D-Galactopyranose was an important intermediate in multiple sugar metabolism pathways of Ophiopogonis Radix， serving as a key to the formation of polysaccharides in Ophiopogonis Radix. During the compatibility process of Rhodiolae crenulatae Radix et Rhizoma and Ophiopogonis Radix， esters， phenols， and carboxylic acids were the key substances that affect their activity.