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食品研究与开发:2025,46(2):178-182
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HPLC 法分析不同提取方法对桦褐孔菌中3 种成分含量的影响
(1.长春师范大学 化学学院,吉林 长春 130032;2.长春师范大学 科技创新研究院,吉林 长春 130032;3.铜仁市疾病预防控制中心,贵州 铜仁 554300;4. 长春师范大学 生命科学学院,吉林 长春 130032)
HPLC Analysis of Effects of Different Extraction Methods on the Content of Three Components in Inonotus obliquus
(1. College of Chemistry,Changchun Normal University,Changchun 130032,Jilin,China;2. Institute of Science and Technology Innovation,Changchun Normal University,Changchun 130032,Jilin,China;3. Tongren Centre for Disease Control and Prevention,Tongren 554300,Guizhou,China;4. College of Life Sciences,Changchun Normal University,Changchun 130032,Jilin,China)
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投稿时间:2023-12-07    
中文摘要: 建立高效液相色谱检测桦褐孔菌粗提物中原儿茶酸、原儿茶醛和紫萁酮3 种化合物含量的方法,对比评价超声辅助提取法、回流提取法、微波辅助提取法、酶解提取法4 种不同提取方法对桦褐孔菌粗提物中活性成分含量的影响。结果表明,该检测方法精密度较高,3 种化合物的精密度的相对标准偏差(relative standard deviation,RSD)分别为0.14%、0.06%、0.20%;重复性RSD 分别为1.67%、1.98%、2.22%;在24 h 内稳定性较好,其RSD 分别为0.20%、0.61%、0.25%。对比不同提取方法对3 种化合物的影响,发现采用超声辅助提取法得到的目标化合物产量较高,适用于桦褐孔菌中主要活性成分的提取。该研究建立的HPLC 法检测桦褐孔菌粗提物中原儿茶酸、原儿茶醛和紫萁酮含量的结果准确。
Abstract:A high performance liquid chromatography(HPLC) method was established to determine the content of protocatechuic acid,protocatechuic aldehyde,and osmundacetone in the crude extract of Inonotus obliquus). The effects of four different extraction methods,i. e.,ultrasonic extraction,reflux extraction,microwave-assisted extraction,and enzyme-assisted extraction,on the content of active ingredients in the crude extract of I. obliquus were compared and evaluated. The results indicated that the assay had high precision,with relative standard deviations (RSD) of 0.14%,0.06%,and 0.20% for the three compounds. It also showed good reproducibility,with RSD values of 1.67%,1.98%,and 2.22%,respectively,and good stability over 24 h,with RSD values of 0.20%,0.61%,and 0.25%,respectively. A comparison of the effects of different extraction methods on the three compounds revealed that the target compounds obtained by ultrasound-assisted extraction gave higher yields and were suitable for the extraction of the main active components in I. obliquus. The HPLC method established in this study for determining the content of protocatechuic acid,protocatechuic aldehyde,and osmundacetone in the crude extract of I. obliquus produced accurate results.
文章编号:202502023     中图分类号:    文献标志码:
基金项目:吉林省科技发展计划项目(20240402045GH)
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