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食品研究与开发:2025,46(2):136-143
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一株波茨坦短芽孢杆菌产胞外多糖条件优化及多糖乳化性分析
(1. 西南民族大学 食品科学与技术学院,四川 成都 610041;2. 四川农业大学 资源学院,四川 成都 611830;3. 四川中烟工业有限责任公司,四川 成都 610021)
Culture Condition Optimization of a Brevibacillus borstelensis Strain for Producing Exopolysaccharides and Analysis of Exopolysaccharide Emulsifying Performance
(1. College of Food Sciences and Technology,Southwest Minzu University,Chengdu 610041,Sichuan,China;2. College of Resources,Sichuan Agricultural University,Chengdu 611830,Sichuan,China;3. China Tobacco Sichuan Industrial Co.,Ltd.,Chengdu 610021,Sichuan,China)
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投稿时间:2023-10-08    
中文摘要: 为提高细菌胞外多糖(exopolysaccharides,EPS)的工业生产效率,该研究通过单因素试验优化一株波茨坦短芽孢杆菌S8 的产糖培养条件,进一步采用Plackett-Burman 设计和最陡爬坡试验对最佳产糖培养条件进行响应面优化,并将菌株所产EPS 分别与不同疏水底物混合,以吐温80 作阳性对照,对其乳化性进行探究。结果表明:优化的最佳产糖条件为接种量4.56%、培养时间39.9 h、碳源添加量30.04 g/L;在此条件下,EPS 产量可达到(107.81±1.47) mg/L;乳化性探究试验结果表明波茨坦短芽孢杆菌S8 产生的EPS 对疏水性底物具有较好的乳化作用,12 h 和24 h 的乳化效果明显优于吐温80。
Abstract:The culture conditions of Brevibacillus borstelensis S8 for producing exopolysaccharides (EPS) were explored by single-factor experiments to improve the production efficiency of EPS. The culture conditions for producing EPS were optimized by Plackett-Burman design and steepest ascent experiments. The EPS produced by strain S8 was mixed with different hydrophobic substrates to investigate its emulsifying performance,with Tween 80 as a positive control. The results showed that the optimal culture conditions of the strain for EPS production were the inoculation amount of 4.56%,carbon source addition at 30.04 g/L,and culture for 39.9 h. Under these conditions,the EPS yield reached (107.81±1.47) mg/L. The EPS produced by B. borstelensis S8 had good emulsifying performance on hydrophobic substrates,with the emulsifying performance better than that of Tween 80 at the time points of 12 h and 24 h.
文章编号:202502018     中图分类号:    文献标志码:
基金项目:卷烟减害降焦四川省重点实验室开放课题项目(川烟工技[2022]220 号);西南民族大学大学生创新创业训练计划项目(S202410656125)
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