Abstract:The extraction process and in vitro functions of the polysaccharide from Fructus mume were studied.The extraction conditions including ultrasonic power，extraction time，solid-to-liquid ratio，and extraction temperature were optimized by response surface methodology.The optimal extraction process was a solid-liquid ratio of 1∶22（g/mL），extraction temperature of 39 ℃，extraction time of 67 min，and ultrasonic power of 160 W.The average yield of polysaccharide extracted from Fructus mume under the optimum conditions was 9.7%，which was close to the predicted value.The results of scanning electron microscopy showed that the extracted polysaccharide appeared a lamellar structure，and the infrared spectrum showed that the polysaccharide was α-morphopyranose.UV results showed that there was no nucleic acid，protein or pigment in the polysaccharide.The results of Congo red test showed that the polysaccharide had no triple helix structure and exerted high antioxidant activity.The polysaccharide at 5 mg/mL showcased the binding rates of 45.73% and 32.50% to sodium glycocholate and sodium taurocholate，respectively. The polysaccharide at 10 mg/mL demonstrated the inhibition rate of 49.7% on pancreatic lipase.The results suggested that Fructus mume polysaccharide had a lipid-lowering activity.