Abstract:The extraction conditions of fucoxanthin from Sargassum were optimized and the antioxidant and anti-inflammatory effects of the purified fucoxanthin were studied in vitro.The results showed that the fucoxanthin extract of Sargassum was stable after storage at 4 ℃in the dark for 25 d.The extraction conditions were optimized by single factor and orthogonal tests as follows：frozen fresh Sargassum as the raw material，90% ethanol mixed with acetone at a volume ratio of 3∶1 as the solvent，solid-to-liquid ratio of 15∶1（g/mL），extraction at 50 ℃and 100 r/min for 6 h，followed by ultrasound-assisted extraction for 15 min.The yield of fucoxanthin reached 0.816 mg/g（wet weight）under these conditions.The extracted fucoxanthin showed the oxygen radical absorbance capacity（ORAC）of 1 761 μmol TE/g and the IC50 values of 0.102 mg/mL and 0.146 mg/mL for scavenging DPPH free radicals and hydroxyl free radicals，respectively.The results of cell experiments showed that extracted fucoxanthin with the concentration below 100 μg/mL had no significant toxicity to mouse small glioma cells（BV-2 cells）.Compared with the model group induced by lipopolysaccharide（LPS），60 μg/mL Sargassum fucoxanthin significantly lowered the nitric oxide（NO）and interleukin-6（IL-6）levels in the cell supernatant.In conclusion，the yield of fucoxanthin extracted from Sargassum was associated with multiple factors，and the extracted fucoxanthin exhibited significant antioxidant and anti-inflammatory effects.