Abstract:Vancomycin-modified magnetic iron oxide nanoparticles（Fe3O4@Van）with photothermal conversion properties were synthesized to establish an integrated platform for rapid detection and photothermal inactivation of Gram-positive bacteria based on chemiluminescence.The carbonyl and amino groups of vancomycin can form hydrogen bonds with peptidoglycan in the cell wall of Gram-positive bacteria，making Fe3O4@Van form complexes with bacteria，which can be captured and extracted from the complexes through magnetic separation.The catalase secreted by bacteria inhibited the luminol -hydrogen peroxide chemiluminescence system.As the number of bacteria in the system increased，the chemiluminescence intensity gradually decreased.This method showed the detection range of 10-105 CFU/mL for Staphylococcus aureus.Meanwhile，due to the excellent photothermal conversion ability of Fe3O4@Van，the laser irradiation at 808 nm can achieve high-temperature inactivation of bacteria in the Fe3O4@Van-bacteria complexes，with the inhibition rate of 98.6% against S.aureus.The novel Fe3O4@Van -based platform provided a new idea for the accurate and efficient detection and inactivation of pathogenic bacteria.