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投稿时间：2021-09-27
投稿时间：2021-09-27
中文摘要: 以紫薯为主要原料，辅以黄豆、花生仁、芝麻籽、玉米、小米、糙米、藜麦、薏米、青稞，选取纤维素酶和复合酶（α-淀粉酶、糖化酶）以三酶两步水解法制备杂粮代餐粉（coarse cereals meal replacement powder，CCMRP）。以葡萄糖当量（dextrose equivalent value，DE值）为指标，通过单因素试验，考察复合酶体积比、酶解时间、酶解温度、复合酶添加量对CCMRP的DE值影响。在单因素试验基础上，通过正交试验对酶解条件进一步优化。研究结果表明，CCMRP酶解条件的影响因素主次顺序：酶解温度＞复合酶添加量＞酶解时间＞复合酶体积比，最佳酶解条件：复合酶体积比1∶2，酶解时间130 min，酶解温度55℃，复合酶添加量0.75%，此时DE值和冲调性的综合分最高，制得的CCMRP冲调性好，色泽、风味、口感俱佳，产品的填充性和流动性有所改善，更利于产品包装。
Abstract:A three-enzyme（cellulase and a compound enzyme composed of α-amylase and glucoamylase）twostep hydrolysis method was employed to prepare the coarse cereal meal replacement powder（CCMRP）with purple sweet potato as the main raw material and supplemented with soybean，peanut，sesame，maize，foxtail millet，brown rice，quinoa，semen coicis，and highland barley.The effects of enzyme volume ratio，enzymolysis time and temperature，and enzyme addition amount on the dextrose equivalent（DE）value of CCMRP were investigated by single factor experiments.On the basis of single factor experiments，the enzymolysis conditions were optimized by orthogonal design.The results showed that the factors affecting the DE value of CCMRP followed the order of enzymolysis temperature＞enzyme addition amount＞enzymolysis time＞enzyme volume ratio.The enzymolysis conditions were optimized as follows:enzyme volume ratio of1∶2，enzymolysis at55℃ for130min，and enzyme addition amount of 0.75%.The CCMRP prepared under these conditions had the highest DE value，fast dissolvability，and excellent color，flavor，and taste.This preparation method improved the filling property and flowability of product，which was conducive to product packaging.
keywords: coarse cereal meal replacement powder multi-enzymatic method enzymolysis condition dextrose equivalent value quality properties
文章编号：202222018 中图分类号： 文献标志码：
基金项目:辽宁省教育厅2021年度高等学校基本科研项目（LJKZ0998）
| Author Name | Affiliation |
| CHEN Yue，GAO Lu* | College of Grain，Shenyang Normal University，Shenyang 110034，Liaoning，China |
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