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投稿时间：2022-04-15
投稿时间：2022-04-15
中文摘要: 以番石榴叶为原料，采用超声波细胞粉碎法辅助提取番石榴叶多糖，探讨各因素对番石榴叶多糖得率的影响，通过Box-Behnken响应面法设计优化番石榴叶多糖的最佳提取工艺。通过测定·OH、DPPH自由基、O2-·的清除能力和总还原力，评价番石榴叶多糖体外抗氧化活性。结果表明，番石榴叶多糖的最佳提取工艺：料液比1∶14（g/mL），超声功率140 W，提取时间16 min，此条件下番石榴叶多糖得率可达（2.00±0.08）%。提取后的番石榴叶多糖具有一定的体外抗氧化活性，其·OH清除率为（23.30±1.29）%，DPPH自由基清除率为（65.43±2.56）%，O2-·清除率为（22.30±1.53）%，具有较强的总还原力。
中文关键词: 番石榴叶 多糖 超声波细胞粉碎 Box-Behnken响应面法 抗氧化
Abstract:Guava leaf polysaccharide was extracted from guava leaves by ultrasonic cell crushing method，and the effects of various factors on the yield of polysaccharide were investigated.Box-behnken response surface methodology was employed to optimize the extraction process of guava leaf polysaccharide.The antioxidant activity of guava leaf polysaccharide in vitro was evaluated based on the·OH，DPPH·，O2-·scavenging abilities and the total reduction capacity.The extraction conditions were optimized as solid-liquid ratio of 1∶14（g/mL），ultrasonic power of 140 W，and extraction for 16 min.Under these conditions，the yield of guava leaf polysaccharide reached（2.00±0.08）%.The extracted guava leaf polysaccharide had antioxidant activity，with the·OH，DPPH·，and O2-·scavenging rates of（23.30±1.29）%，（65.43±2.56）%，and（22.30±1.53）%，respectively，and strong total reduction capacity.
keywords: guava leaf polysaccharide ultrasonic cell crushing Box-Behnken response surface methodology antioxidant
文章编号：202222015 中图分类号： 文献标志码：
基金项目:天津市科技计划项目(21YDTPJC00650)；营养健康关键技术与智能制造（21ZYQCSY00050）
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