Abstract:Active ingredients in a Pueraria extract and tea polyphenols were analyzed by spectrophotometry，and the auxiliary hypoglycemic activity of these compounds was tested in vitro （via inhibitory effect of the α-glucosidase activity）and in vivo(via therapeutic effect on type 2 diabetic mice）.The results showed that the active ingredient content in the Pueraria extract was（51.60±0.45）%and（81.33±0.12）%in tea polyphenols.Their individual semi-inhibitory concentration was 1.000 μg/mL and 0.115 9 μg/mL，respectively.The optimal combination mass ratio was 2∶1，and their semi-inhibitory concentration was 0.133 1 μg/mL and 0.066 55 μg/mL，respectively，when combined.Their combination index was 0.707 3，well under 0.9，thus indicating a good synergistic effect.During the in vivo experiments，400 mg/kg Pueraria extract and 200 mg/kg tea polyphenols were administered to mice，and we observed that both fasting blood glucose and area under curve of glucose（AUC）decreased significantly.Furthermore，the insulin resistance and insulin secretion indexes of these mice improved upon administration of treatment doses.Moreover，the combination of the Pueraria extract and tea polyphenols showed a synergic effect on α-glucosidase inhibition in vitro and a synergic effect on hyperglycemic mice.