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食品研究与开发:2022,43(4):82-88
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超声辅助提取荞麦总黄酮工艺优化及其体外抗氧化活性
(1.内蒙古民族大学生命科学与食品学院,内蒙古 通辽 028043;2.通辽市农牧科学研究所,内蒙古 通辽 028015)
Optimization of Ultrasound-assisted Extraction Technology of Total Flavonoids from Buckwheat and Its Antioxidant Activity in vitro
(1.College of Life Science and Food,Inner Mongolia Minzu University,Tongliao 028043,Inner Mongolia,China;2.Tongliao Institute of Agriculture and Animal Husbandry Science,Tongliao 028015,Inner Mongolia,China)
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投稿时间:2021-03-01    
中文摘要: 该文对超声辅助提取荞麦总黄酮工艺进行优化,同时对通辽地区主要种植的7个荞麦品种的壳粉、全麦粉及芽粉的总黄酮得率、体外抗氧化活性进行测定。在单因素试验基础上进行Box-Behnken试验设计,试验结果表明:超声辅助提取荞麦总黄酮的最优工艺为料液比1∶50(g/mL),提取时间86 min,温度85℃,超声频率72 kHz。7个品种荞麦以珠日河苦荞芽粉中总黄酮得率最高,为(5.13±0.08)%,其DPPH自由基清除率可达(94.79±2.58)%、ABTS+自由基清除率可达(96.92±2.58)%,表明珠日河苦荞芽粉总黄酮的抗氧化能力较强。
中文关键词: 荞麦  总黄酮  提取  优化  抗氧化活性
Abstract:In this paper,ultrasonic assisted extraction of flavonoids was optimized.Total flavonoids yield and in vitro antioxidant activity of the hull,whole-wheat flour,and bud powder of seven buckwheat varieties planted in Tongliao area of China were detected.The Box-Behnken test design performed on the basis of single factor determined the conditions for the optimal ultrasonic extraction of total flavonoids from buckwheat.The optimized conditions were a solid-to-liquid ration of 1∶50(g/mL),extraction time of 86 min,temperature of 85 ℃,and ultrasonic frequency of 72 kHz.After optimization of the seven varieties of buckwheat,the yield of total flavonoids in Zhurihe tartary buckwheat bud powder was the highest(5.13±0.08)%.The DPPH free radical scavenging rate of(94.79±2.58)% and ABTS+free radical scavenging rate of(96.92±2.58)% indicated the strong antioxidant capacity of Zhurihe tartary buckwheat bud flavonoids.
文章编号:202204013     中图分类号:    文献标志码:
基金项目:内蒙古自治区高等学校教学研究项目(NJZY19149);内蒙古自然科学基金研究项目(2019MS03079)
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