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投稿时间：2020-09-14
投稿时间：2020-09-14
中文摘要: 对桂花提取物中黄酮类化合物进行成分鉴定，为进一步开发利用桂花资源提供理论依据。采用乙醇回流法提取桂花中的物质，以芦丁为标准品建立标准曲线，采用NaNO2-A1（NO3）3-NaOH体系络合-分光光度法测定桂花提取物中黄酮类化合物的含量，通过液相色谱-质谱联用技术（liquid chromatograph-mass spectrometer，LC-MS）对桂花提取物中黄酮类化合物进行成分鉴定。结果表明桂花提取物中黄酮类化合物的浓度为3.58 mg/mL，桂花提取物中有miconioside B、egonol gentiobioside、camellianin A等黄酮类化合物的存在，桂花提取物osthin中有miconioside B、negletein 6-[rhamnosyl-（1->2）-fucoside]、kenusanone J等黄酮类化合物的存在。
Abstract:The identification of flavonoids in Osmanthus fragrans Lour.extract provides a theoretical basis for further development and utilization of Osmanthus fragrans Lour.resources.Metabolites were extracted from Osmanthus fragrans Lour.by an ethanol reflux method，and rutin was used to establish a standard curve.The flavonoid content of Osmanthus fragrans Lour.extract was determined using NaNO2-A1（NO3）3-NaOH system complexation and spectrophotometry.Liquid chromatography-mass spectrometry（LC-MS）was used to identify flavonoids in Osmanthus fragrans extract.The concentration of flavonoids in Osmanthus fragrans Lour.extract was 3.58 mg/mL.Flavonoids included miconioside B，egonol gentiobioside，camellianin A.Miconioside B，negletein 6-[rhamnosyl-（1->2）-fucoside]and kenusanone J were found in Osmanthus fragrans Lour.extract osthin.Key words： Osmanthus fragrans Lour.；extract；liquid chromatograph-mass spectrometry （LC-MS）；flavonoids；component identification
keywords: Osmanthus fragrans Lour. extract liquid chromatograph-mass spectrometry （LC-MS） flavonoids component identification
文章编号：202117023 中图分类号： 文献标志码：
基金项目:四川省农业科技成果转化资金项目（15NZ0001）
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