Abstract:The optimum technological conditions for the preliminary separation and purification of total flavonoids from Artemisia argyi was determined.The static adsorption and desorption effects of six macroporous adsorbent resins were compared，and the optimum adsorption resin was determined.The effects of concentration of sample solution，pH value of sample solution，adsorption temperature，sampling speed，elution flow rate and elution dosage on the adsorption and desorption properties of total flavonoids from Artemisia argyi were investigated.Results showed that：AB-8 macroporous resin had the best comprehensive effect.The optimum technological conditions were as follows：the concentration of sample solution was 2.5 mg/mL，the pH value of sample solution was 4，the adsorption temperature was 20℃，the sampling speed was 1.5 mL/min，the elution flow rate was 1.5 mL/min，and the elution dosage was 100 mL.Under the conditions of adsorption and desorption，the purity of total flavonoids from Artemisia argyi from 36.1%to 75.43%.The purity was increased nearly twice，and the purification effect was good.The results of antioxidant test showed that the total flavonoids from Artemisia argyi had certain antioxidant activity，and it was a potential natural antioxidant.