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投稿时间：2019-10-09
投稿时间：2019-10-09
中文摘要: 采用高效液相色谱串联质谱法（high performance liquid chromatography tandem mass spectrometry，HPLC-MS/MS）检测技术测定酸奶中木糖和低聚木糖（木二糖～木六糖）的含量。酸奶样品经乙醇：水（9：1，体积比）提取，采用Altus UPLC BEH Amide（1.7 μm，2.1 mm×100 mm）柱，以乙腈-0.10%氨水溶液为流动相分离目标物。电喷雾质谱负离子模式（electron spray ionization negative，ESI-）下，多反应监测（multiple reaction monitoring，MRM）检测，外标法定量。该方法在0.1 mg/L～5.0 mg/L线性关系良好，相关系数（R>0.999 5），方法检出限在0.12 mg/kg～0.54 mg/kg之间。3个加标水平下进行回收率试验，回收率范围为76.5%～88.4%，相对标准偏差为5.1%～9.8%（n=6）。该方法样品前处理简易、方便，精密度、准确度和灵敏度均满足酸奶中木糖与低聚木糖的检测要求。
中文关键词: 木糖 低聚木糖 酸奶 功能性低聚糖 高效液相色谱串联质谱
Abstract:A method for the determination of xylose and xylo-oligosaccharides in yogurt was established based on the high performance liquid chromatography tandem mass spectrometry（HPLC-MS/MS）.The samples were extractedbyethanol：water（9 ∶1，volume ratio），then separated in an Altus UPLC BEH Amide column（1.7 μm，2.1 mm×100 mm）with binary mobile phase of 0.10% ammonia and acetonitrile.The HPLC-MS/MS with electron spray ionization negative （ESI-）mode was operated and with multiple-reaction monitoring（MRM）mode，the contents of xylo-oligosaccharides were obtained by the external standard method for quantitative analysis.Linearity was observed in the range of 0.1 mg/L to 5.0 mg/L with correlation coefficient higher than 0.999 5，and limits of detection（LODs）was between 0.12 mg/kg and 0.54 mg/kg.The recoveries of xylooligosaccharides at three levels were ranged from 76.5% to 88.4% with the relative standard deviations（RSDs）of 5.1% to 9.8% （n=6）in yogurt.The method was simple，easily and characterized with acceptable sensitivity and accuracy to meet the requirements for the determination of xylose and xylo-oligosaccharides in yogurt.
keywords: xylose xylo-oligosaccharides yogurt functional oligosaccharides high performance liquid chromatography tandem mass spectrometry（HPLC-MS/MS）
文章编号：202019027 中图分类号： 文献标志码：
基金项目:广东省科技计划项目（2016A040403066）；广州市科技计划项目（201806010093）
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