Abstract:Corn resistant starch was prepared by microwave-assisted enzymatic hydrolysis.Based on the yield of corn resistant starch，Box-Behnken test design was carried out on the basis of single factor test.Response surface optimization test analysis was carried out on four factors of high temperature resistance α-amylase addition amount and enzymolysis time，pullulanase addition amount and enzymolysis time.The results showed that the primary and secondary relationship of the four factors was the time of enzymatic hydrolysis of pullulanase，the time of enzymatic hydrolysis of high temperature resistant α -amylase，the amount of high temperature resistant α-amylase and the amount of pullulanase.Response surface optimization test determined the optimal processing parameters of microwave-assisted enzymatic hydrolysis of corn resistant starch：high temperature resistant α-amylase 3 U/g dry starch，enzymatic hydrolysis time 30 min，pullulanase 8 U/g dry starch，enzymatic hydrolysis time 4.5 h.