Abstract:Male mice were intraperitoneal injected with lipopolysaccharide（LPS）/D-galactosamine（D-GalN）to induce acute liver injury after flavonoids of blueberry leaf（FBL）administration.The livers and bloods were collected after LPS/D-GalN injection to measure antioxidative activity and cytokines.The activities of aspartate aminotransferase（AST）and alanine aminotransferase（ALT）in serum were detected to evaluate the functions of the liver.The levels of inflammatory cytokines tumor necrosis factor alpha（TNF-α），interleukin-1 beta（IL-1β），andinterleukin-6（IL-6）weremeasuredusingenzyme-linkedimmunosorbentassay（ELISA）toevaluate the anti-inflammatory activity of FBL.The activities of malondialdehyde（MDA），glutathione（GSH），superoxide dismutase（SOD），and catalase（CAT）in liver tissues were analyzed to evaluate the antioxidative effect of FBL.The activities of AST and ALT were dramatically decreased in the FBL groups，compared with the model mice.FBL significantly repressed the secretion of inflammatory cytokine as a result of LPS/D-GalN administration.FBL treatment efficiently reversed the oxidative insult caused by LPS/D-GalN.The liver alterations induced by LPS/D-GalNwere efficiently relievedby FBLgroup.The resultsindicatedthatFBLpossessed the hepatoprotective effects by inhibiting inflammation and oxidative stress on LPS/D-GalN-induced acute liver injury.