Abstract:Using the shelled peony seed as raw material，the process of ethanol pretreatment of aqueous enzymatic extraction of peony seed oil was optimized.Based on the optimal alkali extraction and enzymatic conditions determined in the previous experiment，the ratio of solid to ethanol，ethanol concentration，temperature and reaction time were optimized by single factor and orthogonal test with free oil extraction ratio as the index in the ethanol pretreatment stage.The optimal conditions of ethanol pretreatment were that material/liquid ratio 1∶8（g/mL），ethanol concentration 80%，temperature 35℃ and reaction time 7 h.Under such conditions，the free oil yield was 90.31%.The prepared peony seed oil has a light yellow color and the solvent residue in accordance with the peony seed oil industry standard.