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食品研究与开发:2020,41(11):118-123
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响应面法优化蛋清蛋白碱法凝胶化条件
(西南大学动物科学学院,重庆402460)
Optimization of Egg Albumin Gelation Process Conditions by Response Surface Methodology
(College of Animal Science,Southwest University,Chongqing 402460,China)
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投稿时间:2019-06-03    
中文摘要: 以蛋清蛋白为原料,添加NaOH制备碱诱导不可逆凝胶。在单因素试验基础上,以NaOH浓度、凝胶化温度和凝胶化时间为自变量,以凝胶强度为响应值,应用响应面法优化蛋清蛋白碱诱导凝胶化条件。试验结果表明:添加NaOH于蛋清蛋白中能制备碱诱导不可逆凝胶。通过建立回归模型,得到了蛋清蛋白碱诱导凝胶化的优化条件:NaOH浓度0.8%,凝胶化温度42℃,凝胶化时间4 h,在此凝胶化条件下蛋清蛋白的凝胶强度预测值为0.936 kgf,验证值为0.933 kgf,二者数值相近,说明得到的优化结果可靠。
中文关键词: 蛋清蛋白  凝胶强度  NaOH  响应面法  回归模型
Abstract:Alkali-induced irreversible gel was prepared by adding sodium hydroxide(NaOH)to the egg albumin which was used as a raw material.On the basis of single-factor experiment,the response surface methodology was used to optimize the conditions of gelatinization induced by egg albumin,in which the NaOH concentration,gelation temperature and gelation time were used as independent variables,and the gel strength was used as the responsevalue.The results showed that sodium hydroxide could be added to egg albumin to prepare alkali-induced irreversible gel.the optimal conditions for egg albumin alkali-induced gelation were obtained as follow through establishment of regression model,sodium hydroxide concentration of 0.8%,gelation temperature at 42℃and the gelation time of 4 h.Under these gelation conditions,the predictive value of gel strength of egg albumin value was 0.936 kgf and the validation value was 0.933 kgf.The two values were similar,which suggests that the optimization results were reliable.
文章编号:202011020     中图分类号:    文献标志码:
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