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投稿时间：2019-04-02
投稿时间：2019-04-02
中文摘要: 香椿营养丰富，风味独特，深受消费者的喜爱，但容易腐烂变质，难以满足市场的需求。为了更好地提高其货架期，本研究以刚刚采摘的香椿嫩芽为试材，采用下一代测序技术比较不同保鲜方法下微生物的群落组成和多样性。结果表明，聚氯乙烯（polyvinyl chloride，PVC）保鲜膜结合紫外照射（ultraviolet irradiation，ZW）处理的香椿较聚氯乙烯保鲜膜、聚氯乙烯保鲜膜结合1-甲基环丙烯（1-methylcyclopropene，1-MCP）、聚氯乙烯保鲜膜结合乙烯吸附剂（ethylene absorbent，EA）处理的香椿的OTUs（Operational Taxonomic Units）和多样性均有所下降，保持优势的细菌门和真菌门分别为蓝菌门（Cyanobacteria）和子囊菌门（Ascomycota）。主成分分析表明，PVC+ZW 处理组的微生物群落相似性更高，群落的稳定性更好。共现网络分析发现，紫外照射提高了不同菌群之间的负相关性。
Abstract:Toona sinensis is rich in nutrition and has unique flavor，which is deeply loved by consumers，however，it is easy to rot and deteriorate，and hard to meet the needs of the market.In order to improve the shelf life of Toona sinensis，the next generation sequencing was used to compare the microbial communities and diversities under different preservation methods.The results showed that the OTUs and diversities of Toona sinensis treated with PVC+ZW were lower than those treated with PVC，PVC+1-MCP，and PVC+EA.The dominant bacteria and fungi were Cyanobacteria and Ascomycota，respectively.Principal component analysis showed that the microbial community of PVC+ZW treatment had higher similarity and better stability.Co-occurrence network analysis showed that ultraviolet irradiation could improve the negative correlation between microbial flora.
keywords: Toona sinensis next-generation sequencing microbial diversity principal components analysis co-occurrence network analysis
文章编号：201914008 中图分类号： 文献标志码：
基金项目:2018 年北京市支农资金农业科技示范推广项目（20180287）；2018 年万人计划教学名师特殊支持项目;天津市林果产业技术体系（ITTFPRS2018009）
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