Abstract:Taking seed oil of Hippophae rhamnoides L.as the research object，the technological conditions of preparation of sterol crude products by saponification method were optimized by single factor and orthogonal test，and sterol crude products were refined and purified by recrystallization method and macroporous adsorption resin method，and identified by thin layer chromatography.The results showed that the optimum technological conditions of preparation of sterol crude products were that solid-liquid ratio was 1∶17.5（g/mL），and solvent 50 % KOH∶anhydrous ethanol ratio was 1∶12.5（Volume ratio），and saponification time was 1.5 h，and saponification temperature was 90 ℃.Under these conditions，the yield rate of sterol crude products was 2.41%.The sterol crude products were refined by recrystallization method，and the sterol refined products containing 91.41 % of β-sitosterol could be obtained.The results of purification by macroporous resin adsorption method showed that the content of β-sitosterol after purification was the highest when the elution time was within 46 min-60 min，which was 93.54 %.The purified sterol of Hippophae rhamnoides L.was β-sitosterol by thin layer chromatography to identity.