Abstract:The response surface method was used to optimize the process conditions and functional characteristics of alkali solubilization and extraction of black soybean protein，and provide theoretical basis for the extraction and utilization of black soybean protein.The response surface analysis was performed by Design-Expert 8.0.6.1 software.The optimized results showed that the ratio of liquid to solid was 12 ∶1（mL/g），pH was 9，extraction temperature was 51℃，and extraction time was 53 min.It is 50.45%.The results of black bean protein functional studies showed that the black soybean protein has a water holding capacity of 2.10 mL/g and an oil holding capacity of 3.15 mL/g，and has good solubility，emulsifying properties，emulsion stability，foaming properties，and foam stability.The optimum process of extracting black soybean protein by alkali dissolution and acid precipitation was determined experimentally to determine the optimal value of black soybean protein functionality，which provided a theoretical basis for the deep processing and product development of black beans.