Abstract:The yellow catfish as the research object，to study the changes in the index at 0，3℃and 6℃ three different temperature group keeping alive without water in the process of survival rate，O2consumption，CO2content，muscle glycogen content，Adenosine triphosphate （ATP）content and lactic acid content，to explore the effect of temperature on yellow catfish keeping alive without water factors.The results showed that the Pelteobagrus fulvidraco survival rate showed a downward trend in the process of keeping alive，keep alive 24 h，0℃the survival rate was 100%，while the 6℃group has dropped to 85.71%degrees.The consumption of O2gradually decreased with the prolonging of preservation time，and the content of CO2increased gradually with the prolonging of living time.The original muscle decreased gradually with prolonged time keeping alive，0℃group decreased the fastest and the slowest 6℃ group.The same trended of ATP and the content of lactic acid，with the extension of the keep alive time increased first and then decreased，in keeping alive before 12 h ATP and lactic acid increased gradually，6℃ group ATP and lactic acid content of the rising rate of the fastest，0℃group ATP and lactate increased rate of the slowest，and keep alive time under the same two groups had significant difference （P＜0.05），12 h ATP and lactic acid content decreased.To sum up，the most suitable Pelteoba－grus fulvidraco keeping alive without water temperature was 0℃.