Abstract:Based on the stability study of human milk vitamins and activity components in cryogenic freezing con－ ditions， the quality standards of Yili Human Milk Physical Library were made to ensure the minimum loss of nu－ trients of the human milk samples stored in Yili Human Milk Physical Library at -80 ℃. The results showed that freezing at -80 ℃ for 12 months significantly reduced vitamins contents ， did not significantly change fatty acid contents nucleotide contents and pH or acidity，compared with fresh milk . VB2 and VA levels were reduced to 35 % and 29 % of fresh breast milk respectively at -80 ℃ for 3 months， and VC was not detected； nicotinic acid was re－ duced to 69 % of fresh breast milk at -80 ℃ for 12 months .Therefore， from the existing test indicators， the quality standards of human milk freezing storage should be based on human milk composition analysed， if stable nutri－ tional ingredients such as fatty acids can be used to store human milk at -80 ℃ for 12 months.