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投稿时间：2012-05-09
投稿时间：2012-05-09
中文摘要: 为了探究脂肪酶水解椰子油的动力学过程，在研究了底物质量浓度、酶添加量、酶解温度及酶解时间对脂肪酶水解椰子油反应速率影响的基础上，本试验采用Lineweaver-Burk法和Wilkinson统计法两种方法对酶解过程进行拟合，计算酶解过程的动力学常数km和Vm，并求解脂肪酶水解椰子油动力学方程。结果表明：在酶添加量为1%、温度为50℃的条件下，动力学常数km为1.2739［mg/（g·mL）］，Vm为0.9696［mg/（g·mL·min）］，。经过试验验证得出米氏方程的拟合度大于0.99，说明方程的预测值与测定值基本吻合，米氏方程适合脂肪酶水解椰子油动力学研究，为油脂酶解过程提供理论模型。
Abstract:A kinetic study on hydrolysis for the production of derivatives catalyzed by Lipase was investigated. The effects of substrate concentration，enzyme concentration，temperature and time on the hydrolysis rate of coconut oil by lipase were analyzed.Based on this，the Michaelis constant（km）and maximum rate（Vm）were deduced by Lineweaver-Burk plotting and Wilkinson statistical method.Results showed that，under the optimal conditions of enzyme concentration 1%and temperature 50℃，the kmand Vmwere 1.273 9 ［mg/（g·mL）］and 0.969 6［mg/（g·mL·min）］respectively..The model-predicted values were consistent with the actually measured values from confirmation tests.Though the simulation of Michaelis-Menten equation to the hydrolysis process，goodness of fit were greater than 0.99，it found that application of Michaelis-Menten equation in hydrolysis process was reasonable.It can provide research foundation for the fat digestion process.
keywords: coconut oil lipase hydrolysis enzymatic kinetics
文章编号：201616017 中图分类号： 文献标志码：
基金项目:国家自然科学基金地区科学基金项目（31160325）；海南省自然科学基金（20153159）；海南省自然科学基金（314075）；海南省高等学校科研项目（Hnky2016ZD-1）
| Author Name | Affiliation |
| WU Lin-he，BAI Xin-peng*，WU Qian，XU Xiao-meng，MA Ruo-ying，LI Xue | College of Food Science and Technology，Hainan University，Haikou 570228，Hainan，China |
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