Abstract:The application of macroporous adsorption resin to purify lentinan，it was found that macroporous adsorption resin DA201-CⅡhad the highest decolorization rate and the lowest polysaccharide retention rate.With decolorization rate as the response value，the influence of amount of lention concentration，addition resin，and decoloration temperature on the process of decolorization were investigated systematically by using one-factor experitments and Box-Behnken design.The results showed that the optimum decolorizing conditions of lentinan by resin DA201-CⅡwere as follows：lention concentration 1.9 mg/mL，addition resin 11 g/100 mL，decoloration temperature 51℃.Under this condition，the decolorization rate and retention rate of polysaccharide were 80.04%and 93.45%.