Abstract:To development a kind of beverage， the main materials were Gomphidius rutilus， jujube and wolf－ berry. Based on sensory evaluation and orthogonal experiments， the progressing technology and the optimum formula of the beverage was discussed. To investigate the anti-fatigue effects of the beverage by employing usual ways in biochemistry of sports. The results indicated that the optimum volume proportion of Gomphidius rutilus， jujube and wolfberry was 2 ∶ 2 ∶ 1， sugar and citric acid were 3 g and 0.15 g， respectively. The beverage had characteristics of beautiful color， sweet and sour taste， and pure flavor. The results showed that the beverage could significantly extend the swimming time of mice， improve the mice hepatic glycogen reserves， reduce lac－ tic acid and urea nitrogen content of the blood. The beverage has anti-fatigue activity.