Abstract:The extraction of silkworm pupa protein with alkali from defatted silkworm pupa powder was studied. Single factor and orthogonal experiments were used to study the effects of the parameters such as temperature ， time，pH and ration of liquid to solid on the yield of silkworm pupa protein. The results showed that the extrac－ tion temperature was the more important factor that affected the effect of extraction，following the ration of liquid to solid and pH and extraction time， the optimal conditions were obtained with orthogonal experiments， as fol lows： extraction temperature was 70 ℃， extraction time was 3.0 h，pH 8.0 and ration of liquid to solid was 14 ∶ 1（mL/g）， under these conditions the yield of silkworm pupa protein was 72.42 %， the protein content of 86.2 %， water content was 11.4 %， the protein was light brown， almost no pupal odor.