Abstract:To establish an efficient，accurate，and sensitive method for detecting deer antler，this study designed three different sets of loop-mediated isothermal amplification（LAMP） primers targeting the mitochondrial gene sequences of sika deer and red deer antler. These primers were used to amplify the target sequences，which were then inserted into the pUC57 vector to construct recombinant plasmids and establish the LAMP detection system. By comparing the amplification effects of different primers and setting a temperature gradient of 61-65 ℃，the detection conditions for sika deer antler and red deer antler were optimized. The sensitivity and specificity of conventional polymerase chain reaction （PCR） and LAMP detection methods were compared，and the optimal detection conditions for commercially available deer antler samples were identified. The results showed that this study successfully established LAMP detection systems for both sika deer and red deer antler，determining the optimal reaction conditions to be incubation at 61 ℃ for 40 min，which allowed for the direct observation of results by the naked eye. Sensitivity experiments showed that the LAMP detection method for sika deer antler had a sensitivity of 2 copies/μL，while the sensitivity for red deer antler was 10 copies/μL，both of which were significantly higher than the results from conventional PCR. Specificity experiments demonstrated that the LAMP system could distinguish between sika deer and red deer antler，making it suitable for detecting commercially available products.