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投稿时间：2020-02-18
投稿时间：2020-02-18
中文摘要: 研究葛仙米藻粉对便秘大鼠的通便作用机理。实验进行4周，随机将大鼠分为对照组、模型组、葛仙米组。对照组连续灌胃生理盐水4周；葛仙米组大鼠连续灌胃0.8 g/kg的葛仙米4周，同时从第4周起葛仙米组灌胃3 mg/kg的盐酸洛哌丁胺；模型组从第1周到第3周灌胃等量生理盐水，第4周灌胃等量盐酸洛哌丁胺。酶联免疫吸附实验法（enzyme-linked immunosorbent assays，ELISA）测定大鼠结肠组织神经递质含量，免疫组化法测定大鼠结肠水通道蛋白表达以及Cajal间质细胞（interstitial cell of Cajal，ICC）标志物c-kit含量。结果显示：（1）模型组大鼠产生首粒黑便的时间显著高于对照组和葛仙米组（p<0.01）。（2）模型组结肠组织5-羟色胺（5-hydroxytryptamine，5-HT）、P 物质（substance P，SP）含量显著低于对照组和葛仙米组（p<0.05），血管活性肠肽（vasoacticeintestinalpolypeptide，VIP）含量显著高于对照组和葛仙米组（p<0.05）。（3）模型组结肠水通道蛋白3（aquaporm3，AQP3）水平显著低于对照组和葛仙米组，水通道蛋白4（aquaporm4，AQP4）水平显著高于对照组和葛仙米组（p<0.05）。（4）模型组结肠ICC细胞c-kit表达显著低于对照组和葛仙米组（p<0.01）。上述结果表明，葛仙米藻粉对便秘大鼠的通便作用，其机理可能与肠神经递质5-HT、SP以及VIP分泌、ICC细胞的结构与功能以及水通道蛋白表达有关。
Abstract:To study the laxative mechanism of Nostoc sphaeroides kiitzing algae powder on constipated rats.The experiment lasted for 4 weeks，and the rats were randomly divided into control group，model group，and Nostoc sphaeroides kiitzing group.The control group was given normal saline continuously for four weeks；the rats in the Nostoc sphaeroides kiitzing group were given continuous intragastric administration of 0.8 g/kg Nostoc sphaeroides kiitzing for 4 weeks，and from the 4th week，the rats in the Nostoc sphaeroides kiitzing group were intragastrically infused with loperamide hydrochloride of 3 mg/kg.The model group was perfused with the same amount of normal saline from the first to the third week and loperamide hydrochloride at the fourth week.ELISA was used to determine the neurotransmitter content in rat colon tissue，and immunohistochemical method was used to determine the expression of aquaporin in rat colon and the ICC marker c-kit content.The results showed：（1）The time for the rats in the model group to produce the first black stool was significantly higher than that of the control group and the Nostoc sphaeroides kiitzing group（p<0.01）.（2）The contents of 5-HT and SP in the colonic tissue of the model group were significantly lower than those of the control group and Nostoc sphaeroides kiitzing group（p<0.05），while the content of vasoactive intestinal peptide VIP was significantly higher than that of the control group and Nostoc sphaeroides kiitzing group（p<0.05）.（3）The level of AQP3 in the model group was significantly lower than that of the control group and the Nostoc sphaeroides kiitzing group，and the level of AQP4 was significantly higher than that of the control group and the Nostoc sphaeroides kiitzing group（p<0.05）.（4）The expression of c-kit in colonic ICC cells in the model group was significantly lower than that in the control group and Nostoc sphaeroides kiitzing group（p<0.01）.The above results indicate that the laxative effect of Nostoc sphaeroides kiitzing on constipated rats may be related to the secretion of enteric neurotransmitters 5-HT，SP and VIP，the structure and function of ICC cells，and the expression of aquaporin.
keywords: constipation Nostoc sphaeroides kiitzing enteric neurotransmitter aquaporin interstitial cells of Cajal
文章编号：202024012 中图分类号： 文献标志码：
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